Abstract
Brucellosis is an endemic disease in China affecting both humans and livestock. The aim of the present study was to analyze two Brucella strains isolated from sheep spleens from Ulanqab in Inner Mongolia, China using classical and molecular typing techniques. The two strains were identified as Brucella suis biovar 3 and were closely related to isolates previously obtained from two different hosts (human and swine) in Guangxi Province. Our results suggest that B. suis can be directly or indirectly transferred from swine to sheep, which act as reservoirs for B. suis infection and later transmitted to humans. Multiple locus variable-number tandem repeat analysis (MLVA) is a useful tool for tracing the geographical origin of brucellosis infections and elucidating its transmission patterns.
Highlights
Brucellosis is a zoonotic bacterial disease caused by Brucella spp., a gram-negative bacteria that infects a wide range of mammals, including domestic and wild animals and humans, resulting in severe economic loss [1, 2]
Multiple locus variable-number tandem repeat analysis (MLVA)-16 showed that these four strains were different but had closely related genotypes (Figure 1)
B. suis biovar 3 has been previously reported as the causative pathogen in human brucellosis in Hainan Province [17], no cases of B. suis biovar 3 infection in animals or humans were observed in Inner Mongolia in past three decades
Summary
Brucellosis is a zoonotic bacterial disease caused by Brucella spp., a gram-negative bacteria that infects a wide range of mammals, including domestic and wild animals and humans, resulting in severe economic loss [1, 2]. It is essential to identify the Brucella species and biovar, which in turn are utilized in epidemiological follow-ups and control of disease. The multiple locus variable-number tandem repeat analysis-16 (MLVA-16) scheme has been extensively utilized in resolving closely related isolates [8, 9]. This method has been proven to be effective in generating epidemiological information on Brucella strains, in identifying its geographical source and elucidating transmission patterns [10, 11]. The MLVA-16 scheme was used to characterize two B. suis isolates from the spleens of sheep from Inner Mongolia that were collected in 2017
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