Molecular imprinting synthetic receptor based sensor for determination of Parkinson's disease biomarker DJ-1

Abstract

Parkinson's disease (PD) is one of the neurological disorders with severe motor and non-motor dysfunction. PARK7/DJ-1 is reported as a highly sensitive PD biomarker protein. So, the DJ-1 protein was chosen as the target protein for the fabrication of a MIP based sensor here. This work reports the fabrication and development of molecularly imprinted polypyrrole (MIPPy) on a screen printed carbon electrode (SPCE) surface for DJ-1 protein recognition.The MIPPy DJ-1 imprinted sensor was carried out by electropolymerization of pyrrole (Py) monomer on a SPCE electrode using cyclic voltammetry (CV) in order to obtain highly selective nano matrices with high molecular recognition capability.Also, the AuNP conjugated impedimetric immunosensor was fabricated for comparison with the MIPPy sensor. MIPPy / immuno sensors were characterized through cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The analytical performance of the MIPPy-based sensor (detection 1 nM; linear range: 1 nM to 500 nM) was comparable with that of the DJ-1 immunosensor (detection limit 1 nM; linear range: 1 nM to 500 nM). These MIPPy/immuno sensors were successfully applied in the DJ-1 analysis in over-expressed and control N2A cells. Also the MIPPy based sensor gave an excellent correlation with AuNP/PPy immunosensor and Western Blot.