Abstract
Based on variation in ribosomal internal transcribed spacer (ITS) DNA a method was developed to identify wood-inhabiting fungi without being dependent on fruit body formation or direct identification of mycelia isolated from wood. A reference library containing restriction fragment length polymorphism (RFLP) profiles of 39 fungal species was constructed by digesting the amplified ITS region with three endonucleases ( Cfo1, Hinf1 and Mbo1). To test the method, early succession of wood-inhabiting fungi was studied in an unmanaged Picea abies forest in Sweden. Fungal species that were present in wood in and at the border of two canopy gaps and a randomly chosen transect of the forest were identified. Bore core samples were taken from 78 standing trees and snags at two gaps and from 30 standing trees positioned in the closed stand. DNA was extracted both directly from wood and from mycelia isolated from wood. Fifteen samples from wood or mycelia from wood were identified as Armillaria borealis, Coniophora arida, Fomitopsis pinicola, Heterobasidion annosum or Resinicium bicolor by comparing RFLP profiles.
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