Molecular Identification of Three Species of Leaf Miners from the Order Diptera on Brassicaceae Crops in Basra Governorate

Abstract

The leafminer (Order: Diptera) is an insect that can be found throughout the Middle East and many other parts of the world. It feeds on a range of plants. It was discovered on several Brassicaceae family leaves in Iraq. In this study, the leafminer samples were collected from the Agricultural Experimental Station – the University of Basrah campus. These samples were microscopically analysed and morphologically characterized to distinguish leafminer genera. Our results revealed that the Agromyzidae family included two species Liriomyza sativae, and Chromatomyia horticola. While the Drosophilidae family involved Scaptomyza flava. These three genera belong to the 420 bases pair. For both intra- and interspecific variation, the mitochondrial region’s COX area has been thoroughly investigated. The results of all these studies combine to create a sizable collection of sequencing data for the Agromyzidae and Drosophilidae families.