Molecular identification of three entomopathogenic fungi infecting the brown plant hopper pest in Indonesia

Abstract

BackgroundBrown plant hopper (Nilaparvata lugens Stal.) a very damaging pest to rice crops. One of the efforts to control it is the use of entomopathogenic fungi (EPF). Three fungal local isolates found in Indonesia were effective in controlling the brown plant hopper pest. This study aimed to molecularly identify the 3 fungal isolates. Molecular identification is very important to get the exact identity of these fungi. The accuracy of EPF identification will greatly determine the success of control. Molecular identification is based on a partial genetic analysis of the internal transcribed spacer (ITS) locus of ribosomal fungal DNA.ResultMorphology of the local isolates named J22 and J60 were identified as Paecilomyces sp., while the isolate J34 was identified as Beauveria sp. The results of molecular identification of the isolates J22 and J60 were identified as the fungi Lecanicillium saksenae and Simplicillium sp., while isolate J34 was identified as Myrothecium sp. The results of literature search showed that the 3 fungi have never been previously reported to infect the brown plant hopper.ConclusionIn Indonesia, 3 types of EPF, namely L. saksenae, Simplicillium sp., and Myrothecium sp., were found having the potential to control the brown plant hopper pest.