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Abstract
Emerging resistance to the antimicrobial agents of choice for treatment of thermophilic Campylobacter infections is becoming a serious threat to public health. In this study, 548 Campylobacter (372 C. jejuni and 176 C. coli) isolates from diarrheal patients and poultry meat were subjected for antibiotic susceptibility analysis to ciprofloxacin, tetracycline, gentamicin, erythromycin and clindamycin. Among them, 151 Campylobacter (32 C. jejuni and 119 C. coli) were identified as multidrug resistant isolates. PFGE analysis was performed on the 151 multidrug resistant isolates to determine their genetic relatedness, and 103 PFGE genotypes were determined. Some isolates from both human and chicken belonged to identical genotypes, indicating these clones might be able to spread between human and chicken. Antibiotic resistant genes of the 151 isolates were identified. The numbers of isolates carried tet (O), aadE, ermB, and aadE-sat4-aphA were 148 (98%), 89 (58.9%), 31 (20.5%), and 10 (6.6%), respectively. Almost all (n = 150, 99.3%) had gyrA mutation at codon 86. And the 23s rRNA A2075G point mutation was found in 56 (37.1%) isolates. Gene mutations at the cmeR-cmeABC intergenic region may lead to the activation of CmeABC multidrug efflux pump, and in this study novel sequence types of the intergenic region were identified in both C. jejuni and C. coli. This study determined the genetic prerequisites for antibiotic resistance of multidrug resistant Campylobacter isolates from diarrheal patients and poultry meat in Shanghai, China.
Highlights
Campylobacter jejuni and Campylobacter coli are major causes of bacterial gastroenteritis in the world (Silva et al, 2011)
The 548 Campylobacter isolates were identified as 372 C. jejuni and 176 C. coli
A large proportion of the 151 isolates were resistant to tetracycline (99.3%), ciprofloxacin (97.4%), erythromycin (80.1%), gentamicin (76.2%) and clindamycin (71.5%)
Summary
Campylobacter jejuni and Campylobacter coli are major causes of bacterial gastroenteritis in the world (Silva et al, 2011). Molecular epidemiology study of C. coli in New Zealand suggested poultry as a main source to the burden of human disease (Nohra et al, 2016). The emergence of antimicrobial resistance to a few common clinical drugs have been repeatedly reported in China (Zhang et al, 2010; Ma et al, 2017) and many other countries (Ge et al, 2013; Narvaez-Bravo et al, 2017). A study on tracking Campylobacter contamination along a broiler chicken production chain in China suggested 71.7% of C. jejuni and 98.0% of C. coli exhibited multidrug resistance (Ma et al, 2014). Our previous study had identified several clones of multidrug resistant Campylobacter from infants younger than 2 years of age (Pan et al, 2015)
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