Molecular Identification of epsA and ompA genes for Acintobacter baumannii Isolated from Burn Wounds

Abstract

This study was designed to molecular detection of epsA and ompA genes for Acinetobacter baumanniiIsolates that isolated from clinical. Burn wounds samples from a total of 80 clinical different Wounds,samples were collected from Ghazi Al Hariri Hospital and Baghdad hospital in Baghdad / Iraq. The resultsof the current study showed that seventy (87.5%) were clinical wounds positive samples, while the rest (10)were negative wounds samples (12.5 %). From 70 samples inoculated on the Chromagar Acinetobacter, 10isolate were grown on the medium, A. baumannii isolates on the Chromagar appeared as bright red coloniesafter 24h and incubation at 37 C . The results of epsA and ompA genes detection clarify that 8 isolate(80%) of A. baumannii isolates gave a positive result and carrying epsA and ompA genes while 2 (20%) ofAcintobacter baumannii isolates were lack the gene and no cross reactivity could be observed with any ofthe AS-3 and AS-9 isolates. The identification of A. baumannii isolates were depended on culturing theseisolates on CHROM agar Acinetobacter, MacConkey, blood, nutrient agar, biochemical tests and API NE 20test VITEK 2 . Compact were carried out the confirms. Results showed the amplification of epsA and ompAgenes for eight. A. baumannii isolates were AS-1, AS-2, AS-4, AS-5, AS-6, AS-7, AS-8 and AS-10. PCRproduct 531 bp of ompA gene and 451 bp of epsA gene.