Abstract
Imipenem-resistant Acinetobacter baumannii (IRAB) represents one of the important causing agents of nosocomial infections especially in immunocompromised and Intensive Care Units (ICUs) patients. The aim of this work was to identify the Imipenem-Resistant genes in Acinetobacter baumannii isolated from Baghdad hospitals. Among 128 A. baumannii isolates, 67 isolates (58.26%) were resistant to imipenem and meropenem. Four genes for imipenem resistance (blaOXA-23 like, blaOXA-24 like, blaOXA-51like and blaOXA-58 like) were amplified and sequenced. The presence of blaOXA-23-like genes in 91.03% of IRAB isolates indicated that the blaOXA-23-like genes are the predominant mechanism for imipenem resistance in our isolates. Sequencing of PCR products showed the presence of new OXA-genes in local A. baumannii isolates including: OXA-207, OXA-239 and OXA-229 among the genes of OXA-24-like, OXA-23-like and OXA-58-like genes, respectively. In conclusion, this study identifies the genes responsible for the imipenem resistance in Baghdad which is important to understand the imipenem resistance and to suggest plans for treatment of patients in future.
Highlights
During the last two decades A. baumannii became clinically important pathogen due to its capability for outbreaks and resistance to most antibiotics including carbapenems [1]
The outbreak of A. baumannii associated with United States military operations in Iraq generated special interest in this organism [2]
Species identification of Acinetobacter baumannii isolates was performed by Polymerase chain reaction (PCR) to detect blaOXA-51-Like genes [11,12]
Summary
During the last two decades A. baumannii became clinically important pathogen due to its capability for outbreaks and resistance to most antibiotics including carbapenems [1]. The outbreak of A. baumannii associated with United States military operations in Iraq generated special interest in this organism [2]. Several studies reported that carbapenem-resistant A. baumannii strains have been emerged around the world [4,5,6]. This resistance is principally caused by the production of carbapenemases [7]. Carbapenem-hydrolysing class D β–lactamases (CHDLs) are the most often reported mechanisms of carbapenem resistance in A. baumannii and four groups of CHDLs have been identified inimipenem-resistant A. baumannii, including intrinsic and chromosomally located OXA51-like β-lactamases and acquired OXA-23-like, OXA-24-like and OXA-58-like β-lactamases [8,9]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
