Abstract
A strain of Acidithiobacillus ferrooxidans designated S t1 was isolated and identified from the acid mine drainage of Tianmashan Coal Mine, Tongling county, Anhui province. Its morphological, physiological and biochemical characters, growth conditions of pH value and temperature, 16S rDNA sequence and the phylogenetic tree was studied. Results showed that the bacteria strains from different sites have divergence at the curve of pH and Eh values during the enriching culture. The isolated strain S t1 grew in a pH rang from 2.0 to 3.0 and temperature 25 to 35°C with over 98％similarity toAcidithiobacillus ferrooxidansin 16S rDNA sequence.
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