Molecular identification and antioxidant test of Chaetoceros sp. from Gondol, Bali

Abstract

Chaetoceros is one of the largest genera of microalgae that has more than 400 species and it is the primary producer in the marine ecosystem. Chaetoceros sp. has some of the pigment which is very important for their survival including chlorophyll and carotenoid pigments. This study aims to identify Chaetoceros sp. from Gondol Bali using ITS fragments, to understand its phylogenetic relationship between microalgae-based on their antioxidant activity. The results of ITS fragments identification and antioxidant activity of Chaetoceros sp. will be used to develop further research related to its potency. The method for microalgae DNA isolation was conducted using the Doyle and Doyle method following with amplification using ITS4 and ITS5 primer. Then, the sequences will be analyzed. The antioxidant activity will be performed by the DPPH method. The results of DNA isolation showed the concentration and purity for about 2842.1 ng / µl and1.97 respectively. PCR products from the amplification of the ITS fragment produced 882 bp. Phylogenetic analysis revealed that Chaetoceros sp. from Gondol Bali had a close relationship with C. muelleri KF 998567.1 and the antioxidant activity test showed IC50 values were about 72,386 ppm.