Abstract
A molecular-genetic identification of four bacterial strains isolated from activated sludge of urban wastewater treatment plants (Ulan-Ude) and the industrial enterprise OJSC “Selenginsky Pulp and Paper Mill” (Selenginsk) was carried out. Bacterial strains were identified by a capillary sequencer ABI 3130XL Genetic Analyzer (Applied Biosystems) using 16S primers 27F and 1492R at the Genomics Collective Use Center of the Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk. The results were obtained using the method of determination of the direct nucleotide sequence of a 16S rRNA fragment followed by comparison of the nucleotide identity with the sequences deposited in the international database GenBank. Bacterial strains isolated from activated sludge were identified according to the GenBank database: strain B 1.1 corresponds to Paenibacillus dendritiformis strain P411 (similarity 99.93%), strains B 1.2 and B 1.3 correspond to Bacillus licheniformis strain PB399 (similarity 86 and 100%, respectively), strain P 1.1 corresponds to the Paenibacillus polymyxa strain ISSDS-85 (similarity 99.86%). The biochemical properties of the identified strains were determined: amylolytic, proteolytic and lipolytic activity; the ability to ferment carbohydrates in Hiss’ nutrient medium; the ability to form ammonia, urea and nitrate reduction. The bacterial strains isolated from activated sludge may be promising for the destruction of wastewater pollutants. On their basis, it is planned to create a consortium of microorganisms for the destruction of protein and fatty pollutants in wastewater.
Highlights
Netic Analyzer (Applied Biosystems) using 16S primers 27F and 1492R at the Genomics Collective Use Center of the Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk
A molecular-genetic identification of four bacterial strains isolated from activated sludge of urban wastewater treatment plants
Bacterial strains were identified by a capillary sequencer ABI 3130XL Ge
Summary
Примечание. «-» – 95 и более процентов отрицательная реакция; «+» – 95 и более процентов положительная реакция, «±» – 50% положительная реакция. 2, штамм B 1.1 по классификатору RDP и GenBank определен как штамм Paenibacillus dendritiformis partial P411, который характеризуется более высоким уровнем вариабельности генов 16S pPHK. Секвенограммы штаммов B 1.2 и В 1.3 при сравнении структур нуклеотидных последовательностей с помощью программы BLAST совпадают между собой и имеют сходство со структурами Bacillus licheniformis strain PB3 chromosome, complete genome и Bacillus licheniformis strain UN1 16S ribosomal RNA gene, partial sequence. Штаммы определили как Bacillus licheniformis strain PB3, который характеризуется более высоким уровнем вариабельности генов 16S рРНК в обоих случаях. 7 показана структура нуклеотидной последовательности штамма P 1.1, полученная с помощью программы BLAST, которая при сравнении совпадает со структурой штаммов Paenibacillus polymyxa strain ISSDS-851 16S ribosomal RNA gene, partial sequence и Paenibacillus sp. 8, идентифицированная нуклеотидная последовательность штамма Р 1.1 по GenBank и классификатору RDP соответствует Paenibacillus polymyxa strain ISSDS-851 16S ribosomal RNA gene, который характеризуется более высоким уровнем вариабельности генов 16S рРНК. Results of the nucleotide sequence identification in the international GenBank database
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