Molecular genetic diversity analysis in short duration pigeonpea [Cajanus cajan (L.) Millsp.

Abstract

In the present study, 48 short duration pigeonpea genotypes were used to analyse molecular diversity employing 20 pigeonpea specific microsatellite markers. Among the 20 microsatellite markers, 11 markers produced polymorphism and nine markers showed monomorphic banding patterns. A total of 35 alleles were generated and the number of alleles produced by different markers ranged from one to three with an average of 1.75 alleles per marker. The polymorphic markers viz., PGM 82, CcM 1615, CcM 2857 and CcM 1277 produced a maximum of three alleles. The allele size ranged from 150 bp (CcLG08_RFQI4) to 290 bp (CcM 1277). The average PIC value obtained was 0.269. The data analysis for microsatellite markers showed high dissimilarity among 48 genotypes. A dendrogram was constructed using Unweighted Pair Group Method with Arithmetic Average (UPGMA) analysis, 48 genotypes were grouped into seven clusters with the similarity coefficient ranged from 0.43 to 1.00. Among the seven clusters, subcluster 1 of cluster I was the largest with fifteen genotypes followed by nine genotypes in the subcluster 2 of cluster II. From this study, it is concluded that the diverse pigeonpea genotypes viz., ICPL 19009, ICPL 19019, ICPL 19030 (cluster I), ICPL 19002 (cluster VI) and CO 6R (cluster VII) could be utilized in the breeding program based on microsatellite marker and cluster analysis. Keywords: Pigeonpea, microsatellite markers, molecular genetic diversity, Polymorphic Information Content, UPGMA clustering.