Abstract
Introduction: Cytokines include different subfamilies such as interleukins (IL), chemokines, and growth factors. They play an important role in inflammatory conditions such as cancer progression and metastasis. There is an increasing interest in developing strategies to antagonize the function of some cytokine/chemokine to interfere with tumor progression and metastasis, the leading cause of death in most patients. The aim of the research project is to study the molecular characteristics of a sample of Syrian patients with breast cancer and assess the protein and gene expression of different inflammatory mediators and correlate that with the clinicopathological criteria of tumors. Materials and methods: Patient samples will be evaluated histologically (H&E stain) and stained immunohistochemically with antibodies against important molecular markers, cytokines, and different types of activated leukocytes. Immunohistochemistry of CD206, a marker of alternatively activated macrophages in tumors is shown here. PCR and immunohistochemical analysis of cytokines (e.g. IL-2, GM-CSF, IFNγ, M-CSG, IL-4, IL-10, CXCL8, CXCL12, CCL21, CCL19, CCR7) will be further implemented. The staining intensity, localization and distribution within the tumor will be examined and correlated with the gene expression and other clinnicopathological information. Expected results: We expect to get new information about the role of different cytokines in breast cancer progression in addition to get an insight into the possible inter-relationship between these cytokines.
Published Version
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