MOLECULAR EVALUATION AND PHENOL APPLICATION EFFECTS ON BARLEY INFESTATION BY Rhyzopertha dominica (F.)

Abstract

Food safety has been a challenge for many years owing to pre and post-harvest losses of agricultural products in the word. In this study, twelve barley cultivars were used to evaluate grain yield, quality parameters and insect pest (Rhyzopertha dominica (F.) infestation using Sequence-Related Amplified Polymorphism (SRAP) marker analysis to study the genetic diversity among the 12 cultivars and Phenol as a chemical application such as modern methods of stored grains protection. SRAP markers confirm that there were highly genetic information differences among all cultivars and their response to insect pest (R. dominica (F.). High polymorphism (83%) and high Polymorphic information content (PIC) (0.84%) was found by primer (me6+em6). The dendrogram of SRAP markers had clustered all the cultivars into two major groups based on their genetic information which could use them in cultivar identification breeding for insect pest control. All the hulless barley had high protein and low phenol content and was more susceptible to R. dominica. Application of the phenol concentration (0.4 g ai/kg) in all the test hulless grain showed high mortality percentages after five days of exposure also caused complete reduction of adult progeny of insect Giza 135 and least damage of grains as lowest Lethal Concentration 50% (LC50%) was 0.33 g ai/kg grain. Moreover, there is no change were found in technological grain properties, thus using phenol will e a positive feature integrated pest management (IPM) programs.