Abstract
BackgroundThe European Regional Office of the World Health Organization (WHO/Europe) developed a strategic approach to halt the indigenous transmission of measles in its 53 Member States by 2015. In view of the goal of measles elimination, it is of great importance to assess the circulation of wild-type measles virus (MV). Genetic analysis is indispensable to understand the epidemiology of measles.MethodsUrine and saliva samples were collected between May 2002 and December 2007, in order to find the origins and routes of wild type measles virus circulation. RT-PCR was performed on a total of 414 clinical samples of patients from different Italian regions. The results confirmed the genome presence in 199 samples, out of which 179 were sequenced. The sequences were genotyped by comparing the fragment coding for the carboxyl terminus of the nucleoprotein (450 nucleotides) with that one of the WHO reference strains.ResultsFrom the year 2002 to the year 2007 phylogenetic analysis of measles sequences showed a predominant circulation of the D7 genotype in the Italian territory for the years 2002–2004. This genotype was replaced by D4 and B3 genotypes in the biennium 2006–2007. During the same period C2, A, D5 and D8 genotypes were also detected.ConclusionsGenetic characterization of wild-type MV provides a means to study the transmission pathways of the virus, and is an essential component of laboratory-based surveillance. Knowledge of currently circulating measles virus genotype in Italy will help in monitoring the success of the measles elimination programme and will contribute to evaluate the effectiveness of future vaccination campaigns.
Highlights
The European Regional Office of the World Health Organization (WHO/Europe) developed a strategic approach to halt the indigenous transmission of measles in its 53 Member States by 2015
Urine and saliva samples were collected between May 2002 and December 2007 (Figure 1) in order to find origins and routes of measles virus (MV) wild-type circulation
The results confirmed the presence of the genome in 199 samples, out of which 179 were sequenced (Table 1)
Summary
The European Regional Office of the World Health Organization (WHO/Europe) developed a strategic approach to halt the indigenous transmission of measles in its 53 Member States by 2015. Interruption of indigenous transmission of measles virus (MV) (Paramyxoviridae; Morbillivirus) has been reported for several countries [4]. Measles remains a leading cause of childhood mortality worldwide, with an estimated 164,000 measles deaths in 2008 (a 78% reduction compared to mortality rate in 2000), most of which took place in developing countries, primarily because of underutilization of the vaccine [5,6]. According to the National Elimination Plan, sensitivity, specificity, and timeliness of case reporting had to be improved. In April 2007 an enhanced surveillance system was established [14], and a National Reference Laboratory (NRL) was established at the Istituto Superiore di Sanità (ISS) in order to support cases ascertainment, confirm outbreaks/cases and determine the MV genotypes
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.