Molecular docking and in vivo immunomodulatory activity of Albizia procera bark on doxorubicin induced immunosuppressive rats

Abstract

ObjectiveTo study the immunomodulatory potential of Albizia procera (AP) bark using in vivo models and by in silico approach. MethodsIn silico models involved to study binding affinity of AP bioactive molecules on immune modified proteins such as Human NF-kappa B p52 (NFkB P52), human tumor necrosis factor-alpha (TNF-α). In vivo studies to evaluated immunomodulatory activity of ethanolic extract AP bark (EEAP) Doxorubicin (DOX) induced immunosuppressive rats. ResultsDocking results showed AP bioactive molecules 3-O-[α-L-arabinopyranosyl-(12)-β- → D fucopyranosyl - (16) - 2 - acetamido - 2 - deoxy- β - → Dglucopyranosyl] echinocystic acid (Compound 1), 3-O-[α-L-arabinopyranosyl-(12)-β- → D fucopyranosyl - (16) - 2 - acetamido - 2 - deoxy- β - → Dglucopyranosyl] acacic acid lactone (Compound 2), Catechin, Quercetin, Isoquercetin were showed immune modulatory activity due to high binding affinity and H bonding interaction with active sites of NFkB P52, TNF-α, without H bonding on anti-inflammatory cytokines IL 10. Based on docking Compound 1, Compound 2, Catechin, Quercetin, Isoquercetin were concluded as immunomodulatory potential candidate. EEAP exhibited a dose related incline in cell count of total leukocyte, neutrophils, and lymphocytes. The suppressive outcome of DOX on these cells was not reflected in EEAP treated rats. It enhanced the rate of clearance of the carbon particles in dose dependent manner from the blood circulation in both normal rats and in the immunosuppressive rats. Delayed type of hypersensitivity test (DTH) results showed an increase in footpad thickness of paw significantly in response to antigen, as an impact of EEAP treatment stimulatory response is observed on lymphocytes along with other essential cells of reaction and thus increased the cell mediated immunity. ConclusionAP improves the immune function in DOX induced immunosuppressive rats.