Abstract
The present research was conducted to isolate and characterize native rhizobium isolates from the root nodules of horse gram [MACROTYLOMA UNIFLORUM [Lam] Verdc.] plants grown under sub-humid to semi-arid regions of Rajasthan in India. These plants were collected from various locations of Rajasthan viz; Banswara, Bhilwara, Pratapgarh and Udaipur districts in India under variable agro-climatic regions. Total nine rhizobial isolates were characterized based on morphological and biochemical analysis. Majority of the isolates showed pH, NaCl concentration and temperature tolerance between 6.0to 8.0, 0.5 to 3.0 per cent and 34 to 40 0C respectively. The horse gram rhizobium isolate R5 showed tolerant towards temperature and NaCl concentration upto 450C and 5% respectively whereas the isolate R3 showed positive growth upto alkaline pH of 10.0. The molecular characterization based on RAPD revealed that rhizobium isolates are closely related to each other as only two major clusters was formed based on Jaccards, s similarity coefficient.
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