Abstract
By the modified ISSR-amplification method the comparison of the structural organization of enzyme loci in the initial common wheat cultivars and their genotrophs induced with epimutagens: plant niacin acid and niacin acid and its derivatives produced from β-picoline fraction of coal-tar pitch (niacin acid nitrile, isocinchomeronic and benzoic acids) was carried out. It is shown that niacin acid influence causes in genotrophs specific band appearance in PCR-profiles obtained on the DNA of enzyme loci. All these before mentioned epimutagens induced in limits of each genotroph differently directed changes in number and intensity of bands of PCR-profiles.
Highlights
In epigenetic investigations, a very important moment is induction of the alterations by means of epimutagens
The established epimutagen is 5-azacytidin causing cytosine demethylation in DNA and activation before inactive genes resulting in alteration of many morphological traits [1,2,3,4,5]
DNA status demethylated under 5-azacytidin action is able to be preserved during cell and sexual generations [1,2,4]
Summary
A very important moment is induction of the alterations by means of epimutagens. The established epimutagen is 5-azacytidin causing cytosine demethylation in DNA and activation before inactive genes resulting in alteration of many morphological traits [1,2,3,4,5]. DNA status demethylated under 5-azacytidin action is able to be preserved during cell and sexual generations [1,2,4]. As it was shown earlier the epigenetic variability could be induced by external factors such as nutrition. Molecular investigations of flax genotrophs demonstrated that inheritable alterations of morphological traits were determined by means of alterations of quantity and structure of DNA in the cell nucleus [8,9]
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