Molecular detection of vicR, vicK and 16S rRNA genes of Streptococcus mutans Isolated from Dental Caries of Iraqi’s Patient

Abstract

Background : This study was carried out to detect the distribution of Streptococcus mutans with dentalcaries in Iraqi’s patient samples. Out of 100 patient’s samples only (22) with age (3-17) years old werefound to be effected with Streptococcus mutans. DNA was extracted amplified by Gradient PCR and thensequenced. vicR was showed highly conserved with no mutation while vicK showed that these were silentmutation and four isolates have missense mutation from eight isolate.Material & Method : In this study, 22 from clinical S. mutans strains were isolated from caries- childrento sequence the vicR, vicK and 16s rRNA genes. Genomic DNA was extracted from S. mutans strains andamplified using Gradient PCR. The PCR products were purified and then sequenced.Result : The molecular study include vicR, vicK and 16S rRNA, genes by Gradient PCR. The result ofsequencing appear that no mutation in vicR genes, although in 16S rRNA present transion and tranvesionmutation . Finally the sequencing of vicK gene appear silent mutation and four isolates have missensemutation from eightConclusion the result of molecular study of vicR gene were appear no mutation in all isolates while 16SrRNA although have transition and transvertion mutation, the Sequence of vicK gene that note more ofmutation was silent mutation but some of them was missense mutation.