Abstract
BackgroundStreptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Sortase is a transpeptidase that anchors several surface proteins to the S. mutans cell wall and has been shown to play a major role in cariogenicity. The purpose of this study was to explore the genetic polymorphisms of the sortase gene (srtA) and the social-behavioural factors associated with dental caries in children with S. mutans.MethodsIn this case–control study, 121 S. mutans strains were separately selected from caries-free children and high-severity caries children for sequencing of the srtA gene. Social and behavioural data were collected by self-administered questionnaires. Genomic DNA was extracted from S. mutans strains and amplified by PCR to obtain the srtA gene. The purified PCR products were sequenced and analysed for mutations with ABI Variant Reporter software. The distribution of missense mutations and the mean of social-behavioural factors were compared between the groups. A multiple logistic regression model was used to control for confounding factors.ResultsThe mutation frequencies at loci 168 (P = 0.023) and 470 (P = 0.032) were significantly different between the groups. The best-fitting model showed that greater age, high frequencies of solid sugar consumption, prolonged breastfeeding, a high proportion of visible plaque, and S. mutans with a T at locus 168 of the srtA gene were associated with high-severity caries in children (P < 0.05). Children carrying a G at locus 168 of S. mutans had a decreased risk for high-severity caries (OR = 0.32, 95% CI = 0.12–0.86) compared with those carrying a T.ConclusionsThe present study suggested that the locus 168 missense mutation of the srtA gene may correlate with caries susceptibility in children with S. mutans. In addition, age, duration of breastfeeding, solid sugar consumption, and poor oral hygiene contributed to this complex disease.
Highlights
Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries
Because an important function of sortase gene (srtA) is the adherence of S. mutans to the tooth surface, we hypothesized that the srtA gene of S. mutans might possess genetic polymorphisms related to different caries conditions
We aimed to explore the genetic polymorphisms of the srtA gene and the social-behavioural factors associated with dental caries in children with S. mutans
Summary
Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Sortase is a transpeptidase that anchors several surface proteins to the S. mutans cell wall and has been shown to play a major role in cariogenicity. The purpose of this study was to explore the genetic polymorphisms of the sortase gene (srtA) and the social-behavioural factors associated with dental caries in children with S. mutans. Socio-demographic variables, development characteristics, general upbringing, oral health related behaviour, oral hygiene and bacteria are known risk factors for the Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries [7]. Sortase A (SrtA), coded by the gene srtA, has been shown to be a membrane-localized transpeptidase that covalently links protein Pac with a sorting signal to the cell wall and possesses important adherent functions that have been associated with cariogenicity [9,10]. Because an important function of srtA is the adherence of S. mutans to the tooth surface, we hypothesized that the srtA gene of S. mutans might possess genetic polymorphisms related to different caries conditions
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