Molecular detection of tungro virus on mekongga and inpari 4 rice varieties in West Papua

Abstract

Tungro disease is one of the important diseases in rice plants. One of the efforts to reduce the spread of tungro virus is to ensure the presence of the virus in the field. This is the first step to prevent the spread and control of tungro disease, especially in West Papua. One detection technique that can be done is molecular detection through PCR techniques. Rice samples detected were Mekongga and Inpari varieties taken from rice plants in West Papua. Total DNA of RTBV, one of the viruses that cause tungro disease, was extracted and amplified using DAF primers (5-GGAATTCCGGCCCTCAaA AACCTAGAAG-3) and DAR (5-GGGGGTACCCCCCTC CGATTTCCCATGTATG-3). The PCR RTBV results showed that the positive samples were infected with Rice tungro bacilliform virus (RTBV). This is indicated by amplification of DNA measuring ± 1400 bp which is the target size of the DAF and DAR primers. The results of this study are preliminary information that can be used as a basis for tungro control and recommendations for future cropping.