Endogenous like sequence Rice Tungro Bacilliform Virus (RTBV) fragments in the local rice genome and its role in controlling tungro disease

Abstract

Tungro disease is one of the important diseases in rice plants because it can cause a large decrease in production. Therefore, tungro disease needs to be controlled in wise ways that ensure environmental sustainability. This can be done by using resistant varieties, while also preventing rice from resistance to the green leafhopper vector Nepothettix virescens, the death of natural enemies, resurgence and emergence of secondary pests which become the main pests. The territory of Indonesia has many very diverse rice germ plasm which its resistance properties can be used to control tungro disease. Resistance genes are an effective control machine especially when combined with other control strategies. One of the triggers for the resistance of rice varieties is eRTBV, which is a group of RTBV nucleotides in rice DNA. The purpose of this study was to detect the presence of the eRTBV piece in the rice genome and its role in controlling tungro disease. The method used wasthe insilico method by comparing the eRTBV found in local rice varieties with that found in Genbank. The results indicated that endogenous RTBV was joined into the rice genome at 1200 bp using the primer eRTBV-7. Nucleotide blast results showed that the similarity of endogenous Indonesian local rice RTBV to endogenous RTBV in the genbank ranged between 95% and 99%, then protein blast results showed that the eRTBV fragment of local rice varieties contained ORF 3 RTBV protein with a range of similarity to the genbank of 66.9% and 93.35%. The role of eRTBV is to produce the Isoleucine protein motif which produces a physical, chemical and biochemical resistance system capable of inhibiting the green leafhopper virus vector (Nepothettix virescens) and the growth and development of tungro disease, RTBV.