Molecular detection of some virulence gene in Acinetobacter spp. isolated from different drinking water samples in AL-Najaf Al-Ashraf Province

Abstract

Contamination of drinking-water is a significant concern for public health throughout the world. Microbial hazards make the largest contribution to waterborne disease in developed and developing countries. Biofilm-producing bacteria were identified by the Congo Red Agar (CRA) method we obtained isolated of Acinetobacter spp. had ability to biofilm formation in medial , the antibiotic resistance screening test to a number of antibiotics on Acinetobacter spp. isolates. The results revealed that isolates of Acinetobacter spp showed a high resistance to amoxicillin, levofloxacin, cefoxitin and imipenem (100 %) followed by amoxicillin and clavulanic and cefoxitin in mediate resistance in Acinetobacter spp , while erthromycin did not show any resistance by Acinetobacter spp. the results indicated that the Ampc gene wasnot present in Acinetobacter baumanni and Acinetobacter lwoffii ,while the results indicated that the MotA gene was presented in Acinetobacter lwoffii as well as the MotB gene was presented in Acinetobacter baumanni and Acinetobacter lwoffii )