Abstract
The present study was carried out to detect the circulating Newcastle disease virus (NDV) from various field outbreaks in Assam during 2018-2021. Nine hundred ninety-two clinical and post mortem samples were collected from the backyard and commercial poultry and subjected to molecular detection by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). NDV could be detected in 445 (n=757, 58.78%) tissue samples and 53 (n=235, 22.55%) cloacal swabs collected from suspected poultry cases by RT-PCR. The findings of our study suggests that the sample of choice for detection of NDV from tissue samples was trachea (n=85, 92.94%), followed by spleen (n=96, 90.62%), lung (n=92, 86.95%), caecal tonsils (n=96, 77.08%), proventriculus (n=74, 60.81%), brain (n=92, 48.91%), intestines (n=73, 31.50%), kidney (n=52, 23.07%), and cloacal swab (n=235, 22.55%) by RT-PCR. Hence, the collection of specific tissue samples from field outbreaks for the detection of NDV is of paramount importance. In addition, RT-PCR can be considered a highly sensitive detection method for rapid and confirmative diagnosis of Newcastle disease virus.
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