Abstract
Foot and mouth disease is infectious, tranboundary, disease of cloven hoofed domesticated ruminants. In this study, ELISA confirmed positive infected and field samples were analyzed through RT-PCR using universal primer set (IF/IR and P1/P2) and RT-LAMP assays. A total of N=100 samples were collected from different livestock population areas in Punjab. Types of samples were serum, nasal secretions, tissue, saliva and swab samples. Out of N=100 infected samples, n=19 samples were serotyped into A, O and Asia 1 strains through antigen ELISA (Ag- ELISA) and these samples were further confirmed through RT-PCR and RT-LAMP assays. ELISA confirmed samples revealed 100% results with RT-PCR and RT-LAMP assays. Field samples revealed 33.33%, 27.16% and 37.03% with RT-PCR (IF/IR and P1/P2) and RT-LAMP assays respectively. It was concluded that ELISA confirmed samples were successfully analyzed with RT-PCR and RT-LAMP assays. Both the assays successfully analyzed 2017 outbreaks of FMD in Punjab.
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