Abstract
Hyposidra talaca is a major defoliating pest of tea plants in north-eastern part of India. In this study, we look for variations (if any) in the attacking virus. Viral samples were collected from different regions of the northern part of West Bengal in India and were analyzed by PCR technique to study the variations across the region. The partial segment of the HytaNPV polyhedrin gene was cloned and sequenced. A 527 bp nucleotide sequence containing highly conserved region from polyhedrin gene of HytaNPV was observed. The blast homology search for studied polyhedrin gene showed 98% sequence identity with the sequence of previous reported NPV of H. talaca, H. infixaria and Buzura suppressaria. Pathogenicity study against second instar H. talaca indicated that the LC50 values ranged from 4.61 × 105 to 7.57 × 105 polyhedral occlusion bodies per ml (POBs/ml) and the LT50 values ranged from 4.2 to 6.66 days. Sequencing result reveals that the same HytaNPV strain dominates across this area and the pathogenicity indicates its potential as an alternative to chemical insecticides to control H. talaca.
Highlights
Baculoviruses are renowned for most widely studied virus of insects
Sequencing result reveals that the same Hyposidra talaca nucleopolyhedrovirus (HytaNPV) strain dominates across this area and the pathogenicity indicates its potential as an alternative to chemical insecticides to control H. talaca
The number of polyhedral occlusion bodies was calculated by a hemocytometer showing the presence of approximately 8.7 9 109 POBs/ml of cadaver
Summary
Baculoviruses are renowned for most widely studied virus of insects. More than 700 baculoviruses have been isolated mainly from the insect species of the orders Diptera, Hymenoptera and Lepidoptera. Nucleopolyhedroviruses (NPVs), which are a part of baculovirus, are considered as potential biocontrol agents and have been applied successfully against larvae of many insect pests in the world (Hu et al 1993; Ma et al 2007). NPVs are mainly pathogenic for insects of Lepidoptera. Polyhedrin gene of NPV is considered as one of the most conserved baculovirus genes (Jehle 2004), and for the development of the genetic amplification technique, this gene proved its efficiency (Woo 2001). The PCR technique is suitable for diagnosis of viruses as identification, Page 2 of 11
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