Abstract
The POU domain is a highly conserved region found in a number of transcription factors and products of developmental control genes. We report here the isolation and characterization of a POU domain-containing cDNA (POU-M1) from the middle silk gland of Bombyx mori. It encodes a protein with a POU domain identical to that of the Drosophila Cf1-a protein. By mobility shift and nuclease protection assays, the POU-M1 protein and the putative silk gland factor 3 (SGF-3) were found to interact in an indistinguishable manner with the SC region (positions -204 to -183) of the sericin-1 gene, a key cis-acting element involved in the regulation of the gene through the interaction with SGF-3. Antibodies raised against the synthetic oligopeptide corresponding to the COOH-terminal region of the putative POU-M1 sequence reacted specifically to both the POU-M1 protein and SGF-3. Northern blot hybridization and Western blotting revealed that POU-M1 expression is regulated both temporally and spatially during silk gland development. We conclude that the POU-M1 protein is SGF-3 and propose that the differential expression of the POU-M1 gene is probably involved in the transcriptional regulation of the silk protein genes.
Highlights
The POU domain is a highly conserved regionf o u n d in decarboxylase gene in selected dopaminergic neurons
We conclude that the POU-M1 protein is silk gland factor 3 (SGF-3) and propose that the differential expression of the POU-M1 gene is probably involved in thetranscriptional regulation of the silk protein genes
Cloning and Sequencing of POU-MI cDNA-A set of fully degenerated primers for the polymerase chain reaction (PCR)were synthesized as described by He et al [3].First strand c D N h were generated from poly(A)+ RNAs of the middle and posterior silkglands of 2-day-old fifth-instarB. mori larvae following standard procedures [17].PCR was performed for40 cycles at
Summary
Cloning and Sequencing of POU-MI cDNA-A set of fully degenerated primers for the polymerase chain reaction (PCR)were synthesized as described by He et al [3].First strand c D N h were generated from poly(A)+ RNAs of the middle and posterior silkglands of 2-day-old fifth-instarB. mori larvae (a Kanebo hybridstrain, Kin-Shu x Sho-Wa) following standard procedures [17].PCR was performed for cycles at. Mori larvae (a Kanebo hybridstrain, Kin-Shu x Sho-Wa) following standard procedures [17].PCR was performed for cycles at. Cloning and Sequencing of POU-MI cDNA-A set of fully degenerated primers for the polymerase chain reaction (PCR)were synthesized as described by He et al [3].First strand c D N h were generated from poly(A)+ RNAs of the middle and posterior silkglands of 2-day-old fifth-instarB.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
