Abstract
Gibberellic acid (GA) signaling regulates diverse aspects of plant growth and developmental processes. The DELLA repressors of GA signaling are named for an N-terminal conserved DELLA domain. In this study, four genes encoding DELLA proteins, PeRGA1, PeRGA2, PeGAI1 and PeGAI2, were isolated and characterized in poplar. A gene structural analysis revealed that the DELLA genes were all intron-free. Multiple protein sequence alignments revealed that these proteins contained seven highly conserved domains: the DELLA domain, the TVHYNP domain, leucine heptad repeat I (LHR I), the VHIID domain, leucine heptad repeat II (LHR II), the PFYRE domain, and the SAM domain. Temporal expression patterns of these genes were profiled during the adventitious root development of poplar. The four DELLA genes were expressed in root, stem and leaf in a dynamic manner. The subcellular localization demonstrated that these DELLA genes were mainly localized to the nucleus. These results suggest that the four DELLA genes may play diverse regulatory roles in the adventitious root, stem and leaf development of poplar, and contribute to improving our understanding of conserved and divergent aspects of DELLA proteins that restrain GA signaling in various species.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-2728-x) contains supplementary material, which is available to authorized users.
Highlights
Gibberellic acid (GA) signaling plays a pivotal role in plant developmental processes and adaptive responses
DELLA proteins contain an N-terminal conserved DELLA domain, which is involved in recognizing GA signaling and interacts with the GA receptor GIBBERELLIN
GA binding to GIBBERELLININSENSITIVE DWARF1 (GID1) stimulates the formation of the GAGID1-DELLA complex, which can induce the degradation of the DELLAs (Davière and Achard 2013)
Summary
Gibberellic acid (GA) signaling plays a pivotal role in plant developmental processes and adaptive responses. Studies have indicated that DELLA proteins negatively regulate the GA signaling pathway and restrain plant growth (Harberd 2003; Achard and Genschik 2009). GA binding to GID1 stimulates the formation of the GAGID1-DELLA complex, which can induce the degradation of the DELLAs (Davière and Achard 2013). The GA-GID1-DELLA complex can interact with an F-box protein. F-box proteins are components of the SCF (SKP1, CULLIN, F-BOX) E3 ubiquitin-ligase complexes, which catalyze DELLA protein degradation through the 26S proteasome, and relieve the inhibitory effect of DELLA proteins on plant growth (Lechner et al 2006). RGA and GAI play important roles in cell division and cell expansion in root, hypocotyl, shoot, and floral induction (Dill and Sun 2001; King et al 2001; Feng et al 2008; Lucas et al 2008; Davière et al 2014).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
