Abstract

This chapter reviews the development of a cDNA probe to the mammalian 28,000 M r calcium-binding protein (CaBP), which has enabled us to study molecular level changes in CaBP in both rat and human tissues for the first time. CaBP is in a position to regulate the levels of free calcium throughout the neuron. For vitamin D deficient rats, weanling male Sprague-Dawley rats from vitamin D-deficient mothers were purchased from Holtzman, Co. and raised for 4 weeks on a vitamin D-deficient diet. A cDNA to mammalian calbindin D 28K has been cloned by direct immunological screening of a λgt-11mouse cerebellar cDNA library. An increase in renal CaBP mRNA is apparent by 1–3 h after hormone treatment followed by a rapid rise in CaBP mRNA. A study of the expression of CaBP mRNA during development indicated that CaBP mRNA in both kidney and brain is present prior to birth but is developmental1y regulated in a tissue specific manner. Recently, a rat Hae III genomic library has been screened using the 1.2 kb CaBP cDNA and two overlapping clones have been isolated and are currently being analyzed.

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