Abstract
This chapter reviews that membranes of Sarcoplasmic Reticulum (SR) and Red Blood Cells (RBC) may have a common gene. Malignant Hyperthermia (MH) is a genetic disorder of skeletal muscle seen in man and pig. Currently, the only reliable screening method for MH is an in vitro halothane contracture test on a biopsied muscle specimen. Halothane is known to cause an abnormal increase of cytoplasmic calcium concentration in skeletal muscle of MH susceptible man and pig. Suspensions of SR or RBC were labelled either by 12-doxylstearic acid (12-DS) or by 16-DS. H-alothane-induced decreases of the order parameter and of the rotational correlation time were more remarkable in MH-RBC than in normal. Differences between MH and normal in both parameters were statistically significant at a halothane concentration of 3 mM. Because a total of 3 ml of blood is sufficient for both measurements and as the blood can be stored with a blood bank preservative for several days without affecting the EPR results, the blood could be shipped to a remote screening center for this noninvasive MH diagnosis, thus making the test widely available.
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