Molecular cloning and functional characterisation of the galactolipid biosynthetic gene TaMGD in wheat grain

Abstract

Monogalactosyl diacylglycerol (MGDG), the main component of the plastid membrane, is essential for chloroplast photosynthesis; however, little information is available about the function of MGDG synthases gene (TaMGD) in wheat grain. In this manuscript, three homologous genes were identified in wheat grain, and their functions were investigated by gene silencing and overexpression techniques. Three TaMGD homologous genes, TaMGD-6A, -6B, and -6D, located on chromosome 6A, 6B, and 6D, respectively, were isolated from common wheat. The transcription of TaMGD was detected in stems, roots, leaves and grains, and high levels of gene transcripts were detected in stems and leaves. Silencing of TaMGD in common wheat spikes resulted in a decrease in grain weight and starch content, and proteomic analysis showed that the differentially expressed proteins mainly included carbohydrate metabolism- and nucleic acid-related proteins. In comparison with wild-type, transgenic rice plants overexpressing TaMGD-6A and -6D showed an increase in thousand kernel weight, as well as an increase in the expression level of genes related to starch biosynthesis, whereas transgenic rice plants overexpressing TaMGD-6B showed increased grain yield and grain number per spike. The results of gene silencing and overexpression indicated that TaMGD plays an important role in wheat grain weight, which might be associated with carbohydrate metabolism. Hence, this study provides new insights regarding the role of TaMGD in wheat grain characteristics.