Abstract
IgD is considered to be a recently-evolved Ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. Although IgD likely plays an important role in vertebrate immune responses, the function of IgD in Nile tilapia (Oreochromis niloticus) is virtually unknown. In the present study, a membrane form of IgD (mIgD) heavy chains were cloned from the GIFT strain of Nile tilapia (designated On-mIgD). The On-mIgD heavy chain’s cDNA is composed of 3347 bp with a 31 bp of 5′-UTR, 3015 bp open reading frame (ORF) and 301 bp 3′-UTR, encoding a polypeptide of 1004 amino acids (GenBank accession no: KF530821). Phylogenetic analysis revealed that On-mIgD heavy chains showed the highest similarity to Siniperca chuatsi. Quantitative real-time PCR (qRT-PCR) analysis showed that On-mIgD expression occurred predominately in head kidney, thymus, spleen, and kidney. After Streptococcus agalactiae infection, transcripts of On-mIgD increased and reached its peak at 48 h in the head kidney and thymus, and 72 h in the spleen, respectively. Taken together, these results collectively indicated that IgD could possibly have a key role to play in the immune response when bacterial infections in Nile tilapia.
Highlights
It is well-known that two heavy chains and two light chains consisting of the Igs, which are important effective molecules in humoral immunity of primates and rodents
In order to study the functions of On-membrane form of IgD (mIgD), we investigated the tissue-specific and response to inactivation of the S. agalactiae expression pattern
In Nile tilapia, On-mIgD mRNA was highly expressed in the head kidney, following the spleen, thymus, and kidney (Figure 5)
Summary
It is well-known that two heavy chains and two light chains consisting of the Igs (immunoglobulins), which are important effective molecules in humoral immunity of primates and rodents. The structures of fish IgD genes have a magical heavy chain, which contain a large amount of constant domains compared with mammals. IgD gene structures of teleosts exhibit high genetic diversity. Afulnthcotiuognhotfhme esmecbreratonreyIgtyDpeinoffisIhgDis hinassusfoficfaiernbt.een reported in catfish (Ictalurus punctatus) [13] and rainbNowiletrtoiluatp(iOa n(Ocorrehoycnhcrhoumsismnyikliostsi)cu[7s)],itsoaouvritkanl ocwomlemdgeerctihael finisfhoramnadticounltruergeadrdailnlgotvheer ftuhnectwioonrlodf, mpaermticburalanrelyIgiDn itnhfieshGuisainngsduoffincgienptr.ovince, southern China. It generally exists in brackNisilhe wtialatperiain(Oersetuocahrrioems iasronuilnotdictuhse) iws oarlvdit[a1l4c].oDmumeetrociOal. finsilhotaicnuds’c(uOlrtueorcehdroamllisonvielorttichues)wroarplidd, pgarrotwictuhlaralnydinhtahredGinueasns,gditonisg cpornovsiidnecree, dsoutothberenaChgionoad. SThanesiemfipnodritnagnst crloelaerilny igninveatme iomreminufnoermreastpioonnsfeo.r the immunoglobulin class diversity in the teleost fish, and suggest t2h.aRteIgsuDltisn tilapia plays an important role in innate immune response Tgheeoifntfhoremfiashtioimn ambuonuet tshyestIegmD.oIfnOt.hneiloptirceussenistlasrtguedlyy,uwnkencolwonne. dStuadmy eomn bthreansetrufoctrumresofantdhediIsgtDribguetinoen ofrfotmhe NIgiDlegteinlaepiina t(iOla.pniail’ostdiciuffse)r, esnutbosregqaunesnwtlyill iennvreicshtigoautredkniotswlteisdsgueeodf itshterifibushtioimn maundneesxypsrteemssi.oInn tchheaprarcetseernisttsictusdiyn, wreespcloonnseed atomeSm. bargaanleacftoiarme oinfftehcetiIognD. gTehneesferomfinNdiilnegtsilapclieaa(rOly. nigloivtiecusm),osruebseinqfuoernmtlaytiionnvesftoirgattehde iitms tmisusuneogdliostbruibliunticolnasasnddivexeprsrietsysiinonthcehateralecotesrtifsitsichs, iannrdesspuogngseesttothSa.taIggaDlacitniateilianpfeiactpiolnay. sThanesiemfipnodritnagnst crloelaerilny igninveatme iomreminufnoermreastpioonnsfeo.r the immunoglobulin class diversity in the teleost fish, and suggest t2h.aRteIgsuDltisn tilapia plays an important role in innate immune response
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