Abstract
The contribution of starch-branching enzyme(EC 2.4.1.18)to starch synthesis in developing sorghum(Sorghum bicolor)grains is very important. In the present work, the cDNA encoding sorghum starch branching enzyme was cloned, sequenced and characterized. This clone was termed SorBE and its accession number in public database is AF169833. SorBE consisted of an open reading frame of 2499 bp encoding a protein of 832 amino acids, with the putative molecular weight of 94.1 kDa. The upstream region of the SorBE mRNA was highly GC rich which caused the difficulty for the 5'-RACE cloning. This SorBE hybridized to mRNA of approximately 2,700 nucleotides whose accumulation was detected in developing sorghum seeds. Southern blot analysis of the sorghum leaf genomic DNA revealed SorBE was encoded by a single gene. Phylogenetic study indicated that this is a seed-specific type I starch branching enzyme.
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