Abstract
Matricaria recutita L. has been known as an important medicinal plant for centuries. The main active components are sesquiterpenes. Squalene synthase (SQS) is an enzyme that plays an important role in regulation of isoprenoid biosynthesis. In this study, the full-length cDNA of the M. recutita SQS gene was cloned and analyzed for the first time. The clone, designated MrSQS (Matricaria recutita L. squalene synthase gene), consisted of 1580 bp, including a 1230-bp-coding sequence for a predicted protein of 409 amino acids with 46.8 kDa molecular weight. MrSQS shows higher homology to the SQS from Artemisia annua than to SQSs from other plants based on its amino acid sequence. Recombinant MrSQS, produced in a bacterial expression system, showed squalene synthase activity in vitro. In M. recutita, the expression level of MrSQS is determined by real-time quantitative PCR that was highest in disk florets and lowest in stems. These results provide basis and foundation for the study and development of regulation of valuable products from M. recutita L.
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