Molecular cloning and characterization of a rice phosphoinositide-specific phospholipase C gene, OsPI-PLC1, that is activated in systemic acquired resistance

Abstract

Phospholipid signaling is an important component in eukaryotic signal transduction pathways; in plants, it plays a key role in growth and development as well as in responses to environmental stress. We cloned and characterized a gene from rice encoding phosphoinositide-specific phospholipase C ( OsPI-PLC1, Oryza sativa L. phosphoinositide-specific phospholipase C1). OsPI-PLC1 encodes a 599 amino acid protein containing the catalytic X and Y domains, as well as a C2-like domain, characteristics of this class of enzymes. Expression of OsPI-PLC1 was induced by various chemical and biological inducers of plant defence pathways, including benzothiadiazole (BTH), salicylic acid (SA), dichloroisonicotinic acid, probenazole (PB), jasmonic acid (JA) and its methyl ester, Pseudomonas syringae pv. syringae, and wounding. All of these treatments were shown to induce resistance in rice against blast disease caused by Magnaporthe grisea. OsPI-PLC1 was activated within 6 h after inoculation with the blast fungus in BTH-treated rice seedlings and in the incompatible interaction between a resistant genotype of rice and M. grisea , whereas the expression in the BTH-untreated seedlings and in the compatible interaction was only induced to a relatively low level at later time points (30 h) after inoculation, indicating that expression of OsPI-PLC1 is associated with the resistance response and/or the incompatible interaction. In addition, BTH treatment also induced systemic expression of OsPI-PLC1. These results suggest that OsPI-PLC1 may be involved in the signaling pathways leading to disease resistance in rice.