Molecular characterization of parents and hybrid progenies of conilon coffee.

Lucimara C De Souza,Aymbiré F.A Da Fonseca,Maria Amélia G Ferrão,Patrícia F Pinheiro,Rodrigo D Carvalho,Taís C.B Soares,Romário G Ferrão

doi:10.1590/0001-3765202120201649

Abstract

The objective of the present work was the molecular characterization of 11 parents and 101 hybrid progenies of conilon coffee, obtained through diallel crosses from the breeding program of the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (Incaper, ES, Brazil). The analyses were performed with 18 Simple Sequence Repeat (SSR) molecular markers, obtaining a total of 32 alleles. SSR markers were classified as moderately informative (PIC = 0.37), being efficient in characterizing individuals. High genetic diversity was verified in the 112 genotypes, based on the greater values of observed heterozygosity about to the expected heterozygosity (0.55 and 0.44, respectively), negative values for the fixation index (F) (-0.14), and the formation of distinct groups by UPGMA. These results indicate high genetic variability among the conilon coffee genitors, which remained similar and persisting in the progenies. The average dissimilarity between parents was 0.29 and between progenies 0.34. The progenies 38 and 40 and the parent P11 were considered the most divergent in the study. The genetic variability found can be explored in the genetic breeding of the conilon coffee and guide crossings between diversified and compatible genetic materials, for the composition of novel cultivars for the state of Espírito Santo.

Highlights

Coffee growing is one of the main agricultural activities of socioeconomic importance in Brazil (Ocde-Fao 2020)
The genetic variability found can be explored in the genetic breeding of the conilon coffee and guide crossings between diversified and compatible genetic materials, for the composition of novel cultivars for the state of Espírito Santo
The genetic materials were obtained from the breeding program of C. canephora, provided by Incaper (Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural – ES, Brazil) in cooperation with Embrapa Café

Summary

Introduction

Coffee growing is one of the main agricultural activities of socioeconomic importance in Brazil (Ocde-Fao 2020). The state of Espírito Santo stands out as the largest Brazilian producer of this species, where it is referred to as conilon coffee (Ferrão et al 2019a). It is characterized as an allogamous crop with self-incompatibility of gametophytic type and great natural variability for different traits (Fonseca 1996, Aguiar et al.2005), as well as high productive potential and tolerance to drought and high temperatures (Ferrão et al 2007). The conilon coffee can be propagated through seeds (sexually) or cuttings (asexually), whereby the consequences of incompatibility on the productivity and genetic variability of the progenies must always be contemplated (Fonseca 1996, Ferrão et al 2007).

Objectives

Methods

Results

Discussion

Conclusion