Molecular characterization of nine grapevine varieties cultivated in Salahaldin, Iraq by using RAPD-PCR marker

Abstract

Grapevine (Vitis vinifera L.) is one of important economic fruit crops found in Salahaldin province \ Iraq. To examine the Molecular characterization and genetic relationships among nine grape varieties by using random amplified polymorphic DNA (RAPD) marker. Fifteen RAPD primers produced polymorphic band (71, 56%), while two RAPD primers yielded monomorphic bands only. The size of the fragment ranged between 250-2700 bp, with an average of 5.47 band/primer. A total of 20 unique and absent bands used to identify seven cultivars. This study has found that genetic distance values ranged from 0.092 to 0.277 among studied grape cultivars. The cluster analysis showed there were two main groups (G1 and G2). These results proposed RAPD marker as rapid, easy and power marker for Molecular characterization and genetic analysis among grapevine.