Molecular Characterization of Human Adenoviruses Associated with Pediatric Respiratory Infections in Omdurman, Sudan

Abstract

Acute respiratory viral infection (ARVI) is a common cause of morbidity and mortality especially in pediatric patients. Among important respiratory pathogens are adenoviruses, The illnesses can range from the common cold to pneumonia Depending on the type, adenoviruses can cause other illnesses such as gastroenteritis, conjunctivitis, cystitis, and, less commonly, neurological disease. Aim of study: molecular detection of respiratory infection due to adenovirus among children in Khartoum state. Respiratory specimens were collected from children’s patients with RTIs in the emergency department Mohamed Alamin Hamid pediatric hospital Omdurman in Khartoum state during the period of October 2022 to February 2023.the HAdV infection were determined by PCR technique. A total of 50 nasal swap were investigated for HAdV, throughout th PCR technique by using specific primer. The results showed 18 (36.0%) were positive and 32 (64.0%) were negative for HAdVs. The statistical analysis revealed no significant difference between age groups (X2= 0.786 P. value: 0.375). This study summarized the HAdV were the predominant types identified in children less than five years intend to have respiratory tract infection according to clinical symptoms of upper or lower respiratory infections.by using conventional PCR for identify with highly sensitively.