Molecular characterization of deep short-axon cells of the rat main olfactory bulb

Abstract

Event Abstract Back to Event Molecular characterization of deep short-axon cells of the rat main olfactory bulb Mark Eyre1*, Katalin Kerti1 and Nusser Zoltan1 1 Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary The most diverse cell populations of neuronal networks are local circuit GABAergic interneurons, and our ability to selectively identify and study them has greatly advanced our understanding of their roles. Interneurons have been classified based on their morphologies, neurochemical content and electrical properties. We recently identified three major subtypes of deep short-axon cells (dSACs) of the main olfactory bulb (MOB) based on their axo-dendritic distributions and synaptic connectivity. Here, we investigated the molecular diversity of dSACs and asked whether distinct dSAC subtypes selectively express certain molecules. Immunofluorescent colocalization experiments revealed that the most commonly used molecular markers of dSACs (i.e. VIP, SOM, CB, NOS) label only very small subpopulations (<7%). In contrast, the voltage-gated potassium channel subunits Kv2.1, Kv3.1b, Kv4.3 and the GABAA receptor α1 subunit are present in 70-95% of dSACs. However, none of these molecules are dSAC-subtype-selective, despite their heterogenous expression levels. Further experiments revealed that mGluR1α labels 22% of the total dSAC population, mostly cells located in the internal plexiform layer. Whole-cell recorded and morphologically identified dSACs immunoreactive for mGluR1α were GL-dSACs, whereas immunonegative dSACs were not. Retrograde tracing of projection dSACs indicated heterogenous expression of several molecules, although none were subtype-selective. Using stereological methods, we estimated the total number of dSACs in the entire MOB to be ~13500, which is around one fourth of the number of mitral cells. Our results provide the first estimate of the total number of dSACs, demonstrate their large molecular heterogeneity and reveal a unique neurochemical marker for one dSAC subtype. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Eyre M, Kerti K and Zoltan N (2009). Molecular characterization of deep short-axon cells of the rat main olfactory bulb. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.052 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Feb 2009; Published Online: 27 Feb 2009. * Correspondence: Mark Eyre, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary, eyre@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Mark Eyre Katalin Kerti Nusser Zoltan Google Mark Eyre Katalin Kerti Nusser Zoltan Google Scholar Mark Eyre Katalin Kerti Nusser Zoltan PubMed Mark Eyre Katalin Kerti Nusser Zoltan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.