Abstract
This experiment was conducted in the Genetics, Breeding and Reproductive Biotechnology Laboratory under Goat and Sheep Production Research Division, Bangladesh Livestock Research Institute (BLRI), Saver, Dhaka, Bangladesh. DNA was extracted from 14 goat breeds and the extracted DNA was observed by gel electrophoresis. Eight goat specific primers were synthesized by ASM-800 DNA synthesizer and screened in the study and all these primers were capable of priming polymorphic amplification pattern in both the breeds. Random amplification of polymorphic DNA - Polymerase Chain Reaction (RAPD-PCR) analysis was carried out using DNA samples of 14 black bengal goat and Jamuna pari goat breeds. Only unambiguous, reproducible and scorable polymorphic fragments were taken into consideration for analysis. Data were analyzed by using a computer programe POPGENE (Version 1.31). Highest level of Nei's (1) gene diversity value (0.4898) was observed in BMS 1494 locus and the mean genetic diversity was obtained 0.3724 among the 14 goat breeds. The highest number of polymorphism obserbed in primer BM1818.The pair-wise genetic distance value ranged from 0.2500 to 1.000. Dendrogram based on Nei's (1) genetic distance using Unweighted Pair Group Method of Arithmetic Means (UPGMA) indicated segregation of the 14 goat breeds. Within Jamuna pari goat genetic similarity is low as well as black Bengal goat.
Highlights
Black Bengal goat is the heritage and pride of Bangladesh
We used Randomly amplified Polymorphic DNA (RAPD) markers for genetic characterization of those goat breeds.19 blood samples were collected from already selected pure Black Bengal goat and Jamuna pari goat from different areas
RAPD amplification of polymorphic DNA-Polymerase Chain Reaction RAPD-polymerase chain reaction (PCR) is a powerful molecular genetic technique for detection of' genetic variability and similarity in the different breeds/population .of, the livestock[21,22]. This technique is highly sensitive to minor alterations in the reaction conditions[23,24] ; optimization of reaction conditions was carried out fur each of the primers by varying certain components of PCR
Summary
Black Bengal goat is the heritage and pride of Bangladesh. It is a dwarf breed found almost in all villages of Bangladesh. Like other domestic goat breeds in South Asia, Bengal goat believed to be derived from wild bezoar of Pasang (Capra aegagrus)[2] with infiltrated blood from markhor (Capra falconeri). Jamuna pari (Nubian type) is another important goat breed in Bangladesh. A molecular marker is a DNA sequence which is readily detected and whose inheritance can be monitored. The use of molecular marker is based on naturally occurring DNA polymorphism, which forms the basis for designing strategies to exploit for applied purpose. A marker must be polymorphic, that is, it must exist in different forms so that chromosome carrying the mutant gene can be distinguished from the chromosome with the normal gene by a marker it carries[4]
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