Abstract
In this study, a novel single-stranded RNA virus was isolated from the plant-pathogenic fungus Colletotrichum higginsianum strain HTC-5, and the virus was named "Colletotrichum higginsianum ssRNA virus 1" (ChRV1). The complete genome of ChRV1 is 3850 nucleotides in length with a GC content of 52% and contains two in-frame open reading frames (ORFs): ORF1 (smaller) and ORF2 (larger). ORF1 encodes a protein with the highest sequence similarity to proteins encoded by Phoma matteucciicola RNA virus 1 (PmRV1, 47.99% identity) and Periconia macrospinosa ambiguivirus 1 (PmAV1, 50.73% identity). ORF2 encodes a protein with a conserved RNA-dependent RNA polymerase (RdRp) domain with similarity to the RdRps of PmRV1 (61.41% identity) and PmAV1 (60.61% identity), which are recently reported unclassified (+) ssRNA mycoviruses. Phylogenetic analysis of the RdRp domain showed that ChRV1 grouped together with PmRV1, PmAV1, and other unclassified (+) ssRNA mycoviruses and had a distant relationship to invertebrate viruses and plant viruses of the family Tombusviridae. This is the first report of a novel (+) ssRNA virus infecting the phytopathogenic fungus C. higginsianum.
Highlights
Mycoviruses have been identified in all major groups of fungal groups [1, 2]
Colletotrichum higginsianum non-segmented double-stranded RNA (dsRNA) virus 1 (ChNRV1), identified in a C. higginsianum strain IMI349063A in United States of America [10], and Colletotrichum higginsianum mitovirus 1 (ChMV1), which we recently reported from a C. higginsianum strain HTC-5
We reported the characterization of a first novel (+) ssRNA mycovirus from C. higginsianum strain HTC-5, which we have provisionally named Colletotrichum higginsianum ssRNA virus 1 (ChRV1)
Summary
Mycoviruses ( called fungal virus) have been identified in all major groups of fungal groups [1, 2]. A novel single-stranded RNA virus was isolated from the plant pathogenic fungus, Colletotrichum higginsianum strain HTC-5, named “Colletotrichum higginsianum ssRNA virus 1” (ChRV1).
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