Abstract
Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from Phoma matteucciicola strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus Penoulivirus (recently proposed) within the family Botourmiaviridae.
Highlights
Mycoviruses infect and replicate in all major filamentous fungal groups, yeasts, and oomycetes [1,2,3]
We reported a novel ourmia-like mycovirus from P. matteuccicola and provisionally named it Phoma matteucciicola ourmia-like virus 1 (PmOLV1)
P. matteuccicola strain LG915-1 was isolated from C. wenyujin, which showed symptoms of leaf blight disease in Hainan province, China, in 2016
Summary
Mycoviruses (fungal viruses) infect and replicate in all major filamentous fungal groups, yeasts, and oomycetes [1,2,3]. In contrast to known plant or animal viruses, mycoviruses usually do not cause any associated symptoms and sometimes even have beneficial effects on their fungal host [4], such as increasing heat tolerance in plant and host fungi [5] and enhancing competitiveness by producing killer proteins in yeasts [6]. Some mycoviruses cause obvious phenotypic alterations including hypovirulence and debilitation, and can be used for biological control of fungal diseases, such as Cryphonectria hypovirus 1 (CHV1) and Sclerotiorum sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) against Cryphonectria parasitica and Sclerotiorum sclerotiorum, respectively [7,8]. The identification of newly isolated mycoviruses may contribute to our understanding of the diversity, evolution, and ecology of viruses [9,10].
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