Abstract
BackgroundMuscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are important etiological agents for Muscovy duck parvoviral disease and Derzsy’s disease, respectively; both of which can cause substantial economic losses in waterfowl industry. In contrast to GPV, the complete genomic sequence data of MDPV isolates are still limited and their phylogenetic relationships largely remain unknown. In this study, the entire genome of a pathogenic MDPV strain ZW, which was isolated from a deceased Muscovy duckling in 2006 in China, was cloned, sequenced, and compared with that of other classical MDPV and GPV strains.ResultsThe genome of strain ZW comprises of 5071 nucleotides; this genome was shorter than that of the pathogenic MDPV strain YY (5075 nt). All the four deleted nucleotides produced in strain ZW are located at the base-pairing positions in the palindromic stem of inverted terminal repeats (ITR) without influencing the formation of a hairpin structure. Recombination analysis revealed that strain ZW originated from genetic recombination between the classical MDPV and GPV strain. The YY strain of MDPV acts as the major parent, whereas the virulent strains YZ99–6 and B and the vaccine strain SYG61v of GPV act as the minor parents in varying degrees. Two recombination sites were detected in strain ZW, with the small recombination site surrounding the P9 promoter, and the large recombination site situated in the middle of the VP3 gene. The SYG61V strain is a vaccine strain used for preventing goose parvoviral disease. This strain was found to be solely involved in the recombination event detected in the P9 promoter region. Phylogenetic analyses between strain ZW and other classical strains of MDPV and GPV were performed. The results supported the in silico recombination analysis conclusion.ConclusionsMDPV Strain ZW is a novel recombinant parvovirus, and the bulk of its genome originates from the classical MDPV strain. Two virulent strains and a vaccine strain of GPV were involved in the recombination process in varying degrees.
Highlights
Muscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are important etiological agents for Muscovy duck parvoviral disease and Derzsy’s disease, respectively; both of which can cause substantial economic losses in waterfowl industry
Sequence comparison of the inverted terminal repeats (ITR) with the pathogenic strains of MDPV and GPV showed that strain ZW shared 97.9% nucleotide similarity with the MDPV strain YY but showed lower similarities with the GPV strain LH (85.7%) and YZ99–6 (86.0%), thereby demonstrating that the ITR of strain ZW originates from the classical MDPV
We presented a detailed description of the genomic characteristics of the novel MDPV strain ZW and confirmed the occurrence of recombination events between GPV and MDPV
Summary
Muscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are important etiological agents for Muscovy duck parvoviral disease and Derzsy’s disease, respectively; both of which can cause substantial economic losses in waterfowl industry. Muscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are etiological agents for Muscovy duck parvoviral disease and Derzsy’s disease, respectively [1]. The viral genomes are approximately 5.1-kb single-stranded DNA with equal polarities encapsidated in the viral capsids [10]. Their genomes are flanked by identical inverted terminal repeats (ITR), which consist of 456 nucleotides for MDPV, and 442 nucleotides for GPV [11, 12]. ITR contains a terminal resolving site (TRS), Rep protein binding site (RBS), and other transcription factor binding sites, which are required for genome replication, transcription, packaging, and rescue from cloning vector [10, 14, 15]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call