Molecular characterization and population genetics of non-CODIS microsatellites used for forensic applications in Brazilian populations

Abstract

Several microsatellites PCR (polymerase chain reaction) multiplex systems (i.e. for simultaneous typing) have been reported for forensic analysis. These include autosomal STR multiplex kits widely used which are commercially available. These commercial kits are generally based on the Combined DNA Index System (CODIS) loci, and a huge volume of genetic population data for the CODIS loci from different ethnic groups has been reported [ 1 Chouery E. Coble M.D. Strouss K.M. Saunier J.L. Jalkh N. Medlej-Hashim M. Ayoub F. Mégarbané A. Population genetic data for 17 STR markers from Lebanon. Leg. Med. 2010; 12: 324-326 Abstract Full Text Full Text PDF PubMed Scopus (13) Google Scholar , 2 Xu L.N. Hu S.P. Feng G.Y. STR polymorphisms of the Henan population and investigation of central plains Han origin of Chaoshanese. Biochem. Genet. 2009; 47: 569-581 Crossref PubMed Scopus (8) Google Scholar , 3 Grattapaglia D. Schmidt A.B. Costa e Silva C. Stringher C. Fernandes A.P. Pereira M.E. Brazilian population database for the 13 STR loci of the AmpfISTR® Profile Plus™ and Cofiler™ multiplex kits. Forensic Sci. Int. 2001; 118: 91-94 Abstract Full Text Full Text PDF PubMed Scopus (26) Google Scholar , 4 Egyed B. Füredi S. Angyal M. Balogh I. Kalmar L. Padar Z. Analysis of the population heterogeneity in Hungary using fifteen forensically informative STR markers. Forensic Sci. Int. 2006; 158: 244-249 Abstract Full Text Full Text PDF PubMed Scopus (21) Google Scholar ]. Nevertheless, there are hundreds of other highly polymorphic STR loci unlinked to the current CODIS loci (non-CODIS markers), which are also useful for forensic genetics [ [5] Asamura H. Ota M. Fukushima H. Population data on 10 non-CODIS STR loci in Japanese population using a newly developed multiplex PCR system. J. Forensic Leg. Med. 2008; 15: 519-523 Abstract Full Text Full Text PDF PubMed Scopus (11) Google Scholar ]. Analysis of further non-CODIS STR loci may complement information from CODIS loci, offering powerful tools for difficult kinship testing, such as sib-ship testing or testing for deficient paternity cases [ [6] Asamura H. Fujimori S. Ota M. Fukushima H. MiniSTR multiplex systems based on non-CODIS loci for analysis of degraded DNA samples. Forensic Sci. Int. 2007; 173: 7-15 Abstract Full Text Full Text PDF PubMed Scopus (26) Google Scholar ].