Abstract
Here, we characterized a negative single-stranded (−ss)RNA mycovirus, Botrytis cinerea mymonavirus 1 (BcMyV1), isolated from the phytopathogenic fungus Botrytis cinerea. The genome of BcMyV1 is 7863 nt in length, possessing three open reading frames (ORF1–3). The ORF1 encodes a large polypeptide containing a conserved mononegaviral RNA-dependent RNA polymerase (RdRp) domain showing homology to the protein L of mymonaviruses, whereas the possible functions of the remaining two ORFs are still unknown. The internal cDNA sequence (10-7829) of BcMyV1 was 97.9% identical to the full-length cDNA sequence of Sclerotinia sclerotiorum negative stranded RNA virus 7 (SsNSRV7), a virus-like contig obtained from Sclerotinia sclerotiorum metatranscriptomes, indicating BcMyV1 should be a strain of SsNSRV7. Phylogenetic analysis based on RdRp domains showed that BcMyV1 was clustered with the viruses in the family Mymonaviridae, suggesting it is a member of Mymonaviridae. BcMyV1 may be widely distributed in regions where B. cinerea occurs in China and even over the world, although it infected only 0.8% of tested B. cinerea strains.
Highlights
Botrytis spp., a group of widespread plant pathogenic fungi, can infect more than 1400 plant species, causing gray mold disease on many economically important crops [1]
Botrytis cinerea mymonavirus 1 (BcMyV1) was clustered with the viruses in the family Mymonaviridae, suggesting it is a member of Mymonaviridae
We describe the genome of a (−ss)RNA virus infecting the fungus B. cinerea, namely Botrytis cinerea mymonavirus 1 (BcMyV1)
Summary
Botrytis spp., a group of widespread plant pathogenic fungi, can infect more than 1400 plant species, causing gray mold disease on many economically important crops [1]. A few sequenced Botrytis viruses, including Botrytis cinerea RNA virus 1 [6], Botrytis ourmia-like virus [7], and Botrytis cinerea negative-stranded RNA virus 1 (BcNSRV1) [8], remained unclassified. Mononegaviruses are a group of nonsegmented (−ss)RNA viruses with the genomes of 8.9–19 kb in length, there are some exceptions [9]. Viruses 2018, 10, 432 virus 1 (SsNSRV1)) is a newly established viral family that accommodates mononegaviruses infecting fungi in the order Mononegavirales [14]. We describe the genome of a (−ss)RNA virus infecting the fungus B. cinerea, namely Botrytis cinerea mymonavirus 1 (BcMyV1). Genomic and phylogenetic analysis indicates that BcMyV1 was most closely related to Sclerotinia sclerotiorum negative-stranded RNA virus 7. We determined the incidence and geographic distribution of BcMyV1 in the population of B. cinerea in China
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