Molecular characterization and expression analysis of an antimicrobial peptide, mytimacin-6, in the small abalone, Haliotis diversicolor

Abstract

Macin is a family of antimicrobial peptides (AMPs) and is involved in the immune responses of marine mollusks. In the present study, a novel Mytimacin (denoted as Hd-mtmc 6) was identified from the small abalone Haliotis diversicolor by RNA-seq and RACE techniques. Hd-mtmc 6 contained a coding sequence of 243 bp and encoded 80 amino acids, with a putative peptide mytimacin 6 consisting of 61 amino acid residues. The mature peptide of Hd-mtmc 6 exhibited typical characteristics of AMPs, including net positive charge (+4), higher hydrophobic residue ratio (37%), and lower molecular mass. Eight cysteines in the mature peptide formed four disulfide bond bridges (C1-C6, C2-C5, C3-C7, and C4-C8). Moreover, the presence of the macin domain, a three-dimensional structure similar to that of hydramacin-1, and a phylogenetic relationship suggested that Hd-mtmc 6 could be a new member of the invertebrate macin family. In unchallenged abalone, the Hd-mtmc 6 transcript was expressed in all tested tissues and could be detected at different stages of embryonic development. Vibrio harveyi challenge caused a significant Hd-mtmc 6 transcripts upregulation within 2 - 6 h in the mantle and hepatopancreas. Our results suggested that Hd-mtmc 6 may be involved in innate immune responses of small abalone. Further investigations are required to confirm its antibacterial activity at the protein level.