Abstract
Background: Paratuberculosis is a chronic infectious disease of wild and domestic ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The present study aimed to conduct the molecular and serological detection of MAP in ruminants of Jabalpur region. Methods: A total of 261 faecal, 124 blood and 51 tissue samples were collected from goats, buffaloes and cattle of the region irrespective of their age, sex and breed. ZN staining of faecal and tissue samples was performed as a preliminary screening test followed by anti-MAP antibodies detection in serum samples using indirect ELISA and IS900 PCR analysis of ZN positive faecal and tissue samples. Result: A pattern of acid-fast bacilli shedding in faecal samples of ruminants was obtained based on ZN staining with a majority of +1 shedders. In ELISA, 33.87% of serum samples were found positive for anti-MAP antibodies whereas, 22.50% ZN positive faecal samples and 100% ZN positive tissue samples were found positive in IS900 PCR. Therefore, a conclusion of moderately prevalent MAP infection in ruminants of the Jabalpur region can be drawn.
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