Molecular and histopathological survey of Francisella spp., Borrelia spp. and Leptospira spp. in ornamental birds of four provinces of Iran

Abstract

Leptospirosis, tularemia, and Lyme disease are among the most important infectious diseases affecting humans, animals, and birds. The present study aimed to identify these infections in exotic birds in Iran using conventional PCR and histopathology methods. The samples consisted of 51 fecal samples and 70 tissue samples collected from 10 different species of ornamental birds in four cities in Iran (Tehran, Tabriz, Ahvaz, and Yazd), each with distinct geographical locations. The presence of bacterial diseases caused by Francisella, Borrelia, and Leptospira in birds was investigated using the 16SrRNA, 5S-23SrRNA, and 16SrRNA genes, respectively. Histopathological examination was conducted on the brain, lung, trachea, kidney, and liver. The results showed a positive rate of 9.91 % for Francisella spp. and 16.52 % for Leptospira spp. DNA in ornamental birds. However, Borrelia species were not identified based on the 5S-23SrRNA gene. Furthermore, the molecular prevalence based on the 16SrRNA gene was 9.9 % (12 out of 121 samples) for Francisella spp. and 16.5 % (20 out of 121 samples) for Leptospira spp. Histopathological studies revealed tracheitis, pneumonia, nephritis, and hepatitis in the PCR-positive birds. To the best of our knowledge, this report represents the first isolation of Leptospira spp. and Francisella spp. from birds in Iran. Considering that both identified bacteria are zoonotic agents, these findings demand a public health risk assessment of exotic birds, which could be important reservoirs for these infections.