MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF STAPHYLOCOCCUS PSEUDINTERMEDIUS FROM CANINE PYODERMA IN SHIVAMOGGA REGION OF KARNATAKA

Abstract

Shivamogga. Exudate/pus/lesion swabs were collected from clinical cases of canine pyoderma (n=126) and subjected to isolation and identification of bacterial isolates by phenotypic methods. The bacteriological processing of the samples resulted in the recovery of 95 staphylococcal isolates and 18 other bacterial isolates. On culture, staphylococci were the most predominantly (n=95, 75.39%) isolated organisms. The PCR was employed as molecular method in this study for the detection of species of staphylococcal isolates by targeting nuc gene and it was also used for the detection of virulence gene and antibiotic resistance gene in staphylococcal isolates by targeting siet gene and mecA gene, respectively, by using primers published earlier. One of the S. pseudintermedius isolates which confirmed by PCR and sequencing of partial nuc gene was used as positive reference strain for further screening of isolates by PCR. Based on nuc gene-based PCR, out of 95 staphylococcal isolates obtained, 82 (86.1%)of the isolates were found belonging to S. pseudintermedius. And out of 82 S. pseudintermedius isolates, siet gene was detected in 69 (86.1%) isolates. S. pseudintermedius was found to be predominant bacterial pathogen responsible for pyoderma in dogs.