Modulatory effect of &lt;i&gt;Althaea officinalis&lt;/i&gt; L root extract on cisplatin-induced cytotoxicity and cell proliferation in A549 human lung cancer cell line

Yukun Zhang,Fanhua Kong,Lei Zhang,Runqi Zhang,Chao Li

doi:10.4314/tjpr.v15i12.16

Abstract

Purpose: To explore the modulatory effect of an Althaea officinalis root extract (AORE) on cisplatininduced cytotoxicity and cell proliferation in a lung cancer cell line. Methods: Aqueous AORE was obtained from peeled and powdered roots. The effect of cisplatin on cytotoxicity and cell proliferation was studied. The cisplatin concentrations tested ranged from 0 - 30 mg/mL. Cell viability and proliferation were studied using trypan blue and MTT assays, respectively. The cells were also exposed to a combination of cisplatin and the AORE. Results: Cisplatin yielded a 50 % inhibitory concentration at 25 mg/mL and exhibited a dose-dependent cell proliferation loss. Combined use of the root extract and cisplatin had significant (R2 = 0.8305) modulatory effects on cytotoxicity and antiproliferative activities. The best inhibitory effects were observed in cells exposed to a combination of 8 or 10 % AORE and 25 mg/mL cisplatin. The optimal effect on cell proliferation was obtained using 25 mg/mL cisplatin and 10 % v/v AORE. Conclusion: The enhanced activity of cisplatin in combination with AORE was more pronounced for cell proliferation than cytotoxicity, indicating that AOREs may be used to control tumor progression and metastasis. Keywords: A549 cells, Althaea officinalis , lung cancer, cell proliferation, cell viability, cisplatin, modulatory effect

Highlights

Althaea officinalis (AO), known as common marshmallow or marshmallow, belongs to the Malvaceae family within the order Malvales
The aim of this work was to determine the effect of AOR on the activity of cisplatin in A549 cells using two end points: cytotoxicity and cell proliferation
Healthy cells did not show any change in morphology when exposed to the AOR extract

Summary

Introduction

Althaea officinalis (AO), known as common marshmallow or marshmallow, belongs to the Malvaceae family within the order Malvales. This plant has perennial roots and stems that die in the autumn [1]. The Greek meaning of the generic name Althaea is “to cure” or “to heal,” signifying the beneficial application of this plant, especially its perennial roots [2]. The roots of the plant are used as a dried powder and as a vegetable. The most common preparation of this plant is marshmallow confectioneries, while the roots of AO are commonly used for their health effects

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